How to Clean a Mattress from Pee (Step‑by‑Step Guide)

David Williams

a close up of a mattress that has been made

Accidents happen—whether it’s a child, pet, or late‑night mishap. The key to cleaning pee from a mattress is acting quickly, using the right cleaning solutions, and eliminating odor, not just stains. This guide explains exactly how to clean a mattress from pee, both fresh and dried, using safe and effective methods.


What You’ll Need

Most of these items are likely already at home:

  • Paper towels or clean cloths
  • Baking soda
  • White vinegar
  • Spray bottle
  • Mild dish soap or laundry detergent
  • Hydrogen peroxide (3%)
  • Cold water
  • Vacuum cleaner
  • Optional: enzyme cleaner (best for pet urine)

How to Clean Fresh Pee from a Mattress

Step 1: Blot Immediately

  • Use paper towels or a dry cloth
  • Press firmly to absorb as much liquid as possible
  • Do not rub—this pushes urine deeper into the mattress

Step 2: Apply Vinegar Solution

Mix in a spray bottle:

  • 50% white vinegar
  • 50% cold water

Spray generously over the affected area. Vinegar neutralizes ammonia and kills odor‑causing bacteria.

Let it sit for 10–15 minutes.


Step 3: Blot Again

Blot the area until it’s mostly dry.


Step 4: Cover with Baking Soda

  • Sprinkle a thick layer of baking soda over the spot
  • Let it sit for 8–12 hours (overnight is best)

Baking soda absorbs moisture and odor.


Step 5: Vacuum Thoroughly

Vacuum all baking soda residue. The mattress should now be clean and odor‑free.


How to Clean Dried Pee from a Mattress

Dried urine requires a deeper clean.

Step 1: Rehydrate the Stain

Lightly spray the area with cold water (don’t soak it).


Step 2: Use a Cleaning Solution

Option A: Vinegar Solution

  • Same 50/50 vinegar and water mix
  • Spray and let sit for 15 minutes

Option B: Hydrogen Peroxide Solution (for tough stains)
Mix:

  • 1 cup hydrogen peroxide (3%)
  • 3 tbsp baking soda
  • A few drops of dish soap

Spray lightly and let it fizz.
⚠️ Test first—this can slightly lighten fabric.


Step 3: Blot and Dry

Blot until damp, not wet.


Step 4: Baking Soda Treatment

Cover the area and let sit overnight, then vacuum.


Best Method for Pet Pee (Highly Recommended)

Use an enzyme cleaner designed for urine (especially for cats and dogs).

Why enzyme cleaners work:

  • Break down urine proteins
  • Permanently remove odor
  • Prevent repeat marking

Follow the product instructions exactly for best results.


How to Remove Urine Smell Completely

If odor remains:

  • Repeat baking soda treatment
  • Increase air circulation with a fan
  • Place mattress in sunlight if possible (UV kills bacteria)
  • Never use steam cleaners—heat sets urine odors

How Long Should the Mattress Dry?

  • Surface dry: 2–4 hours
  • Fully dry internally: 12–24 hours

✅ Use a fan
✅ Open windows
❌ Do not use a hair dryer (can trap odor)


Prevent Future Accidents

  • Use a waterproof mattress protector
  • Wash bedding regularly
  • Train pets consistently
  • Address medical issues (children or pets)

A quality mattress protector is the easiest long‑term solution.


Common Mistakes to Avoid

  • Using hot water (sets stains)
  • Oversaturating the mattress
  • Skipping odor neutralization
  • Covering mattress before fully dry
  • Using harsh chemicals like bleach

Final Thoughts

Knowing how to clean a mattress from pee can save you money, time, and stress. With quick action and the right cleaning method, even strong urine odors can be completely removed.

For best results:

  • Act fast
  • Neutralize odors
  • Let the mattress dry fully